Part:BBa_K4759202:Design

T7-RBS3-OleP

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 47
  
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 47
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 47
  Illegal AgeI site found at 103
  Illegal AgeI site found at 201
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

is selected to synthesize the above gene by adding histidine tags at the C-terminus through E. coli codon optimization and subcloning the above genes onto the plasmid pET-28a to obtain the recombinant plasmid pET-28a-oleP.

Source

is from the oleandomycin synthesis pathway of Streptomyces antibioticus.